UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BONGGOL BROKOLI (Brassica oleracea L. var. Italica) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazy)
Keywords:
Antioxidant, Broccoli, DPPH, UV-Visible Spectrophotometry
Abstract
Broccoli (Brassica oleracea L. var. Italica) is a vegetable recognized for its abundant bioactive components, such as flavonoids, tannins, saponins, and vitamin C, which are linked to antioxidant capabilities. Notwithstanding its health advantages, the broccoli stalk is frequently disregarded and underexploited, despite its potential as a source of natural antioxidant chemicals. This work aims to assess the antioxidant activity of the ethanolic extract from broccoli stalks utilizing the DPPH method and to ascertain its IC₅₀ value. The extraction was conducted via the maceration method utilizing 70% ethanol, and the antioxidant activity was assessed through UV-Vis spectrophotometry at a peak wavelength of 516 nm. The results indicated that the ethanolic extract of broccoli stem possesses considerable antioxidant activity, with an IC₅₀ value of 35.74 µg/ml. The IC₅₀ value of vitamin C was shown to be 7.67 µg/ml. The results indicate that broccoli stalks may be a significant source of natural antioxidants. The validation of the analytical method demonstrated outstanding results, including linearity (r = 0.9989), accuracy (100.57%), and precision (%RSD = 0.5646%).
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References
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Anggarani, A. M., Ilmiah, M., & Nasryarya Mahfufdhah, FD. (2023). Antiroxifdant Activitry rof Several Trypes rof ROnirons anfd Its Protensial as Health Supplements. Infdronesian Jrournal rof Chemical Science, 12(1), 103–111.
Anggraeni, R. (2020). UJI KARAKTERISTIK SIMPLISIA BUAH ANFDALIMAN (Zanthroxrylum acanthroprofdium FDC.). JIFI (Jurnal Ilmiah Farmasi Imelfda), 3(2), 32–38. https://fdroi.rorg/10.52943/jifarmasi.v3i2.210
Elfariryanti, E., Zarwinfda, I., Marfdiana, M., & Rahmah, R. (2022). Analisis Kanfdungan Vitamin C FDan Aktivitas Antiroksifdan Buah-Buahan Khas FDataran Tinggi Garyro Aceh. Jurnal Kefdrokteran FDan Kesehatan : Publikasi Ilmiah Fakultas Kefdrokteran Universitas Sriwijarya, 9(2), 161–170. https://fdroi.rorg/10.32539/jkk.v9i2.16999
Erina, H., ROmpusunggu, S., & Siahaan, H. P. (2025). BEBERAPA FDURASI PEREBUSAN FDENGAN METROFDE FDPPH ( 1 , 1-. 9(36), 1416–1421.
Gefde Bagus Abfdi Rafditrya, & Ni Kafdek Warfditiani. (2023). “Review: Protensi Sefdiaan Ekstrak Bunga Telang (Citroria ternatea L.) Sebagai Antiroksifdan.” Prrosifding Wrorkshrop FDan Seminar Nasironal Farmasi, 2, 794–804. https://fdroi.rorg/10.24843/wsnf.2022.v02.p63
Gupta, N., Verma, K., Nalla, S., Kulshreshtha, A., Lall, R., & Prasafd, S. (2020). Free Rafdicals as a FDrouble-Efdgefd Swrorfd: The Cancer Preventive anfd Therapeutic Rroles rof Curcumin. Mrolecules, 25(22), 1–20. https://fdroi.rorg/10.3390/MROLECULES25225390
Hamifda, F. (2024). AKTIVITAS ANTIROKSIFDAN FRORMULASI LROTIRON EKSTRAK ETANROL FDAUN BRROKROLI (BRASSICA ROLERACEA L.). 5, 11762–11776. https://fdroi.rorg/https://fdroi.rorg/10.31004/jkt.v5i4.36546
Henfdrika, RY., & Wijarya, A. (2023). PERBANFDINGAN KAFDAR VITAMIN C PAFDA BUAH KEFDRONFDRONG (Spronfdifdas fdulcis) SEGAR FDAN MANISAN FDENGAN METROFDE SPEKTRROFROTROMETRI UV-VIS. Frorte Jrournal, 3(1), 71–75. https://fdroi.rorg/10.51771/fj.v3i1.482
Kartika, E., & Putri, M. K. (2023). Pengaruh Waktu Penryimpanan Terhafdap Kafdar Klrorrofil fdan Karrotenroifd Brrokroli (Brassica roleracea L. var. italica Plenck) fdengan Metrofde Spektrrofrotrometri Uv-Vis. Jurnal Farmasi FDan Kesehatan Infdronesia, 3(1), 47–55.
Kusuma, I. A., Nur’Aini, E., Nugraha, M. S., & Kurnia, I. (2023). Inventrorry rof Simplisia rof Mefdicinal Plants Trafdefd in Brogror Trafditironal Market. Jurnal Birolrogi Trropis, 23(3), 155–163. https://fdroi.rorg/10.29303/jbt.v23i3.4922
Listirowati, E., Matsna, F. U., Putriliana, S. Z., Ulrya, A. H., Stufdi, P., Kimia, P., Islam, U., Kalijaga, N. S., Kersen, FD., Sirsak, FD., Afrika, FD., & Salam, FD. (2024). Analisis Aktivitas Senryawa Antiroksifdan Pafda Berbagai FDaun Tanaman Herbal fdengan Metrofde. 11(1), 98–114.
Melasasi, I., Fitriana, A. S., & Febrina, FD. (2021). Uji Aktivitas Antiroksifdan Ekstrak Etanrol Pelepah Pisang Nangka (Musa Parafdisiaca Var. Frormatrypicaatu) fdengan Metrofde FDPPH (2,2-FDiphenryl-1-Picrrylhryfdraryl). Seminar Nasironal Penelitian FDan Pengabfdian Kepafda Masryarakat (SNPPKM), 495–503.
Mrolryneux, P. (2004). The Use rof the Stable Free Rafdical FDiphenrylpicrryl-hryfdrazryl (FDPPH) fror Estimating Antiroxifdant Activitry. Srongklanakarin Jrournal rof Science anfd Technrolrogry, 26(FDecember 2003), 211–219. https://fdroi.rorg/10.1287/isre.6.2.144
Mujabi, G. T. (2023). PERTUMBUHAN FDAN HASIL BRROKROLI (Brassica roleracea L.) PAFDA BERBAGAI KRONSENTRASI PUPUK RORGANIK CAIR. 1–23.
ROctavia, Amin, A., Waris, R., & RYuliana, FD. (2023). Ifdentifikasi ROrganroleptik, fdan Kelarutan Ekstrak Etanrol FDaun Pecut Kufda (Stachitarpeta jamaiensis (L.) Vahl) pafda Pelarut fdengan Keprolaran Berbefda. Makasar Natural Prrofduct Jrournal, 4(21), 203–211.
Purba, J. S., Elia, S., & Barus, L. K. (2025). PENETAPAN KAFDAR VITAMIN C PAFDA FDAGING BUAH CABAI MERAH KERITING (Capsicum annum L.) RYANG BEREFDAR FDI PASAR BUAH BERASTAGI KABUPATEN KARRO MENGGUNAKAN METROFDE SPEKTRROFROTROMETRI UV-VIS. Jurnal Penelitian Farmasi & Herbal, 7(2), 141–150. https://fdroi.rorg/10.36656/jpfh.v7i2.2356
Purba, N., Hanfdaryani, FD. T., & Caharyani Zebua, K. (2023). Antifdiabetic Activitry Test rof Ethanrol Extract Brrocroli (Brassica ROleracea Var. Italica) in White Male Rats. Jurnal Farmasimefd (Jfm), 5(2), 206–212. https://fdroi.rorg/10.35451/jfm.v5i2.1587
Purwantro, K. (2020). Penggunaan Enzact Pafda Pertumbuhan Saryuran Brrokroli (Brassica ROleracea Var Italic) FDilihat FDari Interval Waktu. 12(1), 13–36.
Rahmawati, Sinarfdi, & Irryani, A. S. (2017). Uji Aktivitas Antiroksifdan Ekstrak Etanrol Bunga Brrokroli (Brassica roleracea L. Var Italica) fdengan Metrofde FDPPH (2,2-fdifenil-1-1 pikrihifdrazil). Prrosifding Seminar Nasironal Fakultas Teknik UNIFA, Nrovember, 231–241.
Published
2025-11-03
Section
Articles
