FORMULASI DAN EVALUASI AKTIVITAS ANTIOKSIDAN FACE MIST BUAH BIT MERAH (BETA VULGARIS L.) DENGAN METODE DPPH

Abstract

Red beetroot (Beta vulgaris L.) contains antioxidant compounds such as flavonoids and betalain which have the potential to be used as active ingredients in cosmetic preparations. This study aimed to formulate red beetroot ethanol extract into a face mist preparation, evaluate the physical characteristics of the preparation, and determine its antioxidant activity using the 2,2 diphenyl -1-picrylhydrazyl (DPPH) method. This study was an experimental study with a face mist formulation that contains red beetroot ethanol extract at a concentration of 5% (F1), 10% (F2), and 15% (F3), as well as a formula without extract (F0) as a control. The evaluation included organoleptic test, homogeneity, pH, viscosity, spray power, dry time, and antioxidant activity based on the IC50 value.The study results show that all face mist formulas meet the requirements of physical evaluation of the preparation. Antioxidant activity testing showed consecutive IC50 values of 289,19 µg/mL (F0), 69,83 µg/mL (F1), 63,82 µg/mL (F2), and 51,59 µg/mL (F3). The higher the concentration of red beet fruit extract used, the stronger the antioxidant activity produced. F3 formula with 15% extract concentration provides the best antioxidant activity and is classified as strong, close to the very strong category. Based on the study results, it can be concluded that red beetroot ethanol extract (Beta vulgaris L.) has been successfully formulated into a face mist preparation that meets the requirements of physical quality and has good antioxidant activity. 15% extract concentration is the most effective formulation as an antioxidant. It is suggested that researchers can further develop red extract (Beta vulgaris L.) in other dosage forms and increased concentrations.