FORMULASI DAN EVALUASI SEDIAAN GEL MOISTURIZER EKSTRAK ETANOL DAUN KIRINYUH (Chromolaena odorata L.) TERHADAP BAKTERI Propionibacterium acnes

Abstract

Kirinyuh leaves (Chromolaena odorata L.) contain secondary metabolites, including flavonoids, alkaloids, tannins, saponins, steroids, and phenolic compounds, which have potential antibacterial activity against Propionibacterium acnes. This study aimed to formulate the ethanolic extract of kirinyuh leaves into a moisturizer gel, evaluate its physical quality and stability, and determine its antibacterial activity against Propionibacterium acnes. The extract was obtained by maceration using 96% ethanol and formulated into three concentrations of 15%, 20%, and 25%. The formulations were evaluated for organoleptic properties, homogeneity, pH, spreadability, viscosity, cycling test, and storage stability for two weeks. Antibacterial activity was determined using the disk diffusion method. The results showed that all formulations met the physical evaluation requirements and exhibited good stability. All formulations inhibited the growth of Propionibacterium acnes, with Formula F3 (25%) producing the largest inhibition zone. In conclusion, the ethanolic extract of kirinyuh leaves can be formulated into a moisturizer gel that meets the required physical quality standards, is stable, and possesses antibacterial activity against Propionibacterium acnes.