PENENTUAN KANDUNGAN ANTIOKSIDAN DAN FENOLIK TOTAL DARI EKSTRAK TUMBUHAN SEBAGAI BIOREDUKTOR DALAM PEMBENTUKAN NANOPARTIKEL PERAK
Keywords:
Silver nanoparticles, orthophenanthroline, Folin-Ciocalteu, antioxidant
Abstract
The formation of metal nanoparticles (silver, gold, platinum) by biological methods (green synthesis) has advantages over chemical and physical methods because it is easier, faster, simpler, and environmentally friendly. One of the substances used in this method is plant extracts. The presence of secondary metabolites that act as antioxidants such as phenolics and flavonoids play roles in the formation of silver nanoparticles. In this study, phytochemical screening was carried out from several herbal plants as bioreductors in the formation of silver nanoparticles, such as Phyllanthus buxifolius, Pachira aquatica, Peperomia pellucida, Ageratum conyzoides, and Piper crocatum leaf extracts. The total antioxidant content was determined using the modified phenanthroline method, and the total phenolic content determined using the Folin-Ciocalteu method. The formation of silver nanoparticles was carried out by mixing silver nitrate solution with each plant extract. Colloidal silver nanoparticles formed were then measured for their absorption spectra. The results of the antioxidant content of the five consecutive samples were 26,90 ± 0,19; 26,09 ± 0,14; 18,25 ± 0,02; 42,76 ± 0,14; dan 30,94 ± 0,14 mg AA/g, while the total phenolic contents were 48,44 ± 0,45; 21,08 ± 0,92; 17,42 ± 0,27; 57,71 ± 0,47; dan 49,83 ± 0,60 mg GA/g. Silver nanoparticles mediated by Ageratum conyzoides leaf extracts provided the highest absorbance value compared to other plants. The antioxidants and phenolic contained in the extract acts as reducing agent from silver ions into silver nanoparticles.
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References
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Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8(4). https://doi.org/10.3390/PLANTS8040096
Granja, C., Jara, E., Moncada-Basualto, M., Pozo-Martínez, J., Fernández, L., Espinoza-Montero, P., Debut, A., López, F., Olea-Azar, C., & Pilaquinga, F. (2021). Total Phenolic Composition and Antioxidant Activity of Silver Nanoparticles using Aqueous Extract of Chilca Leaves (Baccharis Latifolia). https://doi.org/10.3844/ajassp.2021.79.91
Gurning, K., Lumbangaol, S., Situmorang, R. F. R., & Silaban, S. (2021). Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method. Jurnal Pendidikan Kimia (JPKIM), 13(2), 137–142. https://doi.org/10.24114/jpkim.v13i2.26984
Hanifa, D., Sarina, G., & Djamaan, A. (2020). Toona sinensis Mediated Green Synthesis of Silver Nanoparticles. IOSR Journal Of Pharmacy And Biological Sciences (IOSR-JPBS) e-ISSN, 15(2), 2319–7676. https://doi.org/10.9790/3008-1502040815
Hastuti, S., Safitri, I. A., Poltekkes, P. F., & Mulia, B. (2015). Aktivitas Analgetik Ekstrak Etanol Daun Sligi (Phyllanthus buxifolius Muell .Arg ) terhadap Mencit Galur Balb/C (Analgesic Activity Of Ethanol Extract Seligi Leaves (Phyllanthus buxifolius Muell .Arg) to Mice Balb/C). Indonesian Journal on Medical Science, 2(1), 2355–1313. http://ejournal.poltekkesbhaktimulia.ac.id/index.php/ijms/article/view/9
Hussain, Z., Abourehab, M. A. S., Khan, S., & Thu, H. E. (2020). Silver nanoparticles: a promising nanoplatform for targeted delivery of therapeutics and optimized therapeutic efficacy. Metal Nanoparticles for Drug Delivery and Diagnostic Applications, 141–173. https://doi.org/10.1016/B978-0-12-816960-5.00009-4
Jalab, J., Abdelwahed, W., Kitaz, A., & Al-Kayali, R. (2021). Green synthesis of silver nanoparticles using aqueous extract of Acacia cyanophylla and its antibacterial activity. Heliyon, 7(9). https://doi.org/10.1016/J.HELIYON.2021.E08033
Labulo, A. H., David, O. A., & Terna, A. D. (2022). Green synthesis and characterization of silver nanoparticles using Morinda lucida leaf extract and evaluation of its antioxidant and antimicrobial activity. Chemical Papers, 76(12), 7313–7325. https://doi.org/10.1007/S11696-022-02392-W/TABLES/4
Lister, I. N. E., Ginting, C. N., Girsang, E., Armansyah, A., Marpaung, H. H., Sinaga, A. P. F., Handayani, R. A. S., & Rizal, R. (2019). Antioxidant properties of red betel (Piper crocatum) leaf extract and its compounds. Journal of Natural Remedies, 19(4), 198–205. https://doi.org/10.18311/JNR/2019/23633
Mukaratirwa-Muchanyereyi, N., Gusha, C., Mujuru, M., Guyo, U., & Nyoni, S. (2022). Synthesis of silver nanoparticles using plant extracts from Erythrina abyssinica aerial parts and assessment of their anti-bacterial and anti-oxidant activities. Results in Chemistry, 4, 100402. https://doi.org/10.1016/J.RECHEM.2022.100402
Musa Özcan, M., Doğu, S., & Uslu, N. (2018). Effect of species on total phenol, antioxidant activity and phenolic compounds of different wild onion bulbs. https://doi.org/10.1007/s11694-017-9705-0
Nagaich, U., Gulati, N., & Chauhan, S. (2016). Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract.https://doi.org/10.1155/2016/7141523
Pahmi, K., Ramadhian, M. R., Dalimunthe, G. I., Mikrobiologi, D., Parasitologi, D., Kedokteran, F., Lampung, U., & Lampung, K. B. (2022). Antipyretic Activity Test of Combination of Peperomia pellucida Extract and Andrographis paniculata in Mice (Mus musculus). Indonesian Journal of Pharmaceutical Education, 2(3), 187–196.https://doi.org/10.37311/IJPE.V2I3.15654
Raja, S., Ramesh, V., & Thivaharan, V. (2017). Green biosynthesis of silver nanoparticles using Calliandra haematocephala leaf extract, their antibacterial activity and hydrogen peroxide sensing capability. Arabian Journal of Chemistry, 10(2), 253–261. https://doi.org/10.1016/J.ARABJC.2015.06.023
Refilda, R., Ngestu, R. H., Salim, E., & Yefrida. (2022). Teknik Edible Coating dengan menggunakan Campuran Gel Lidah Buaya dan Ekstrak Daun Psidium guajava L. untuk Mempertahankan Sifat Fisikokimia Buah Jambu Biji. Jurnal Riset Kimia, 13(2), 163–177. https://doi.org/10.25077/JRK.V13I2.501
Rodrigues, A. P., & Pastore, G. M. (2021). A review of the nutritional composition and current applications of monguba (Pachira aquatica Aubl.) plant. Journal of Food Composition and Analysis, 99, 103878. https://doi.org/10.1016/J.JFCA.2021.103878
Sarwer, Q., Amjad, M. S., Mehmood, A., Binish, Z., Mustafa, G., Farooq, A., Qaseem, M. F., Abasi, F., & Pérez de la Lastra, J. M. (2022). Green Synthesis and Characterization of Silver Nanoparticles Using Myrsine africana Leaf Extract for Their Antibacterial, Antioxidant and Phytotoxic Activities. Molecules 2022, Vol. 27, Page 7612, 27(21), 7612. https://doi.org/10.3390/MOLECULES27217612
Setyawati, A., Wahyuningsih, M. S. H., Nugrahaningsih, D. A. A., Effendy, C., Fneish, F., & Fortwengel, G. (2021). Piper crocatum Ruiz & Pav. ameliorates wound healing through p53, E-cadherin and SOD1 pathways on wounded hyperglycemia fibroblasts. Saudi Journal of Biological Sciences, 28(12), 7257–7268. https://doi.org/10.1016/J.SJBS.2021.08.039
Urnukhsaikhan, E., Bold, B. E., Gunbileg, A., Sukhbaatar, N., & Mishig-Ochir, T. (2021). Antibacterial activity and characteristics of silver nanoparticles biosynthesized from Carduus crispus. Scientific Reports, 11(1), 1–12. https://doi.org/10.1038/S41598-021-00520-2/FIGURES/10
Yefrida, Y., Suyani, H., Aziz, H., & Efdi, M. (2020). Validasi Metode MPM untuk Penentuan Kandungan Antioksidan dalam Sampel Herbal serta Perbandingannya dengan Metode PM, FRAP dan DPPH. Jurnal Riset Kimia, 11(1), 24–34. https://doi.org/10.25077/JRK.V11I1.3
Published
2023-05-31
Section
Articles
