- Antifungal Potential of Nanosimplicia Gummy Candy Formulated from Pineapple Stem (Ananas comosus) and Melon Seeds (Cucumis melo L.) Against Candida albicans–Induced Oral Ulcers
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Kata Kunci:
Candida albicans, Nanosimplisia, Batang nanas, Biji Melon, Gummy candyAbstrak
Kandidiasis oral merupakan suatu infeksi yang disebabkan oleh jamur Candida albicans. Agen antijamur yang umum digunakan di masyarakat sebagian besar merupakan bahan kimia sintetis. Penggunaan bahan kimia sintetis dalam jangka panjang sering menimbulkan efek negatif, seperti resistensi jamur dan efek samping sistemik lainnya. Metabolit yang terdapat pada batang nanas berperan sebagai agen antijamur, yaitu saponin, flavonoid, tanin, alkaloid, senyawa fenolik, serta enzim bromelain. Bromelain merupakan campuran enzim proteolitik yang pertama kali ditemukan pada batang nanas. Biji melon mengandung metabolit tanin, flavonoid, dan saponin, serta alkaloid, terpenoid, protein, dan karbohidrat. Senyawa metabolit yang terdapat pada kedua limbah bahan alam tersebut dalam klasifikasi biofarmasetika tergolong ke dalam BCS kelas II (kelarutan rendah dan bioavailabilitas tidak stabil). Hal ini menyebabkan zat aktif sulit terlarut dalam cairan fisiologis. Untuk mengatasi permasalahan tersebut, dikembangkan teknologi nanosimplisia inovatif dalam bentuk sediaan gummy candy. Penelitian ini diawali dengan formulasi nanosimplisia dari batang nanas dan biji melon dengan variasi waktu sonikasi, yang selanjutnya diformulasikan ke dalam sediaan gummy candy dengan perbandingan (0:0,5; 0,5:1; 1:1,5). Selanjutnya dilakukan uji aktivitas antijamur terhadap Candida albicans. Hasil penelitian menunjukkan bahwa formulasi terbaik diperoleh dari kombinasi kedua nanosimplisia dengan perbandingan 1:1,5 yang menunjukkan zona hambat sebesar 30,23 ± 0,06 mm pada konsentrasi 750 ppm.
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