COMPARISON OF ANTIOXIDANT ACTIVITY OF FRESH AND DRIED BANGUN-BANGUN (Plectranthus Amboinicus L.) LEAF DECOCTIONS USING THE DPPH METHOD BY UV–VIS SPECTROPHOTOMETRY
Kata Kunci:
Bangun-Bangun Leaves, Antioxidant, DPPH MethodAbstrak
Bangun-bangun (Plectranthus amboinicus L.) is an ethnobotanical plant traditionally used by the people of North Sumatra, especially for postpartum mothers. This plant contains secondary metabolites such as flavonoids, alkaloids, tannins, saponins, and steroids, which have antioxidant potential. Antioxidants play an important role in neutralizing free radicals that can cause oxidative stress and degenerative diseases. This study aimed to determine the antioxidant content, IC50 value, and antioxidant activity of fresh and dried bangun-bangun leaf decoctions based on IC50 values. This research used a quantitative experimental design. Antioxidant activity was analyzed using the DPPH method with a spectrophotometer, showing a maximum DPPH wavelength of 517 nm. Phytochemical screening of bangun-bangun leaf powder confirmed the presence of alkaloids, tannins, saponins, flavonoids, and steroids. The results showed that the fresh leaf decoction had an IC50 value of 94.622 µg/mL, classified as strong antioxidant activity, while the dried leaf decoction had an IC50 value of 102.33 µg/mL, classified as moderate antioxidant activity. Vitamin C, used as a positive control, showed very strong antioxidant activity with an IC50 value of 10.291 µg/mL
Referensi
Ambarati, T., Wahyudi, N. Y., Hamidah Asmara Indratno, S., Nurfadhila, L., & Utami, M. R. (2023). Article Review: Validation of Analytical Methods for Determining Paracetamol Levels in Biological Samples Using Various Methods. Journal of Pharmaceutical and Sciences, 6(2), 838–847. https://doi.org/10.36490/journal-jps.com.v6i2.157
Anggreini D., Zebua, N. F., Hidayat, S., Ningrum, S. R., Nadia, S., & Yanti, F. (2024). Antioxidant Activity Test and Determination of SPF Value of Bangun-Bangun Leaf Extract (Plectranthus Amboinicus (Lour.) Spreng) in Facial Serum Preparations. Miracle Journal 4(2).
Arviani., et al. (2023). Pharmacognosy: Exploring the Secrets of Medicine from Nature.
Aspan, R., et al. (2008). Taxonomy of Medicinal Plant Collections at the Citeureup Medicinal Plant Garden. Indonesian Food and Drug Administration.
Aziz, S. (2017). Cultivation of Bangun-Bangun. October 2013, 1–3.
Binuni, R., Maarisit, W., Hariyadi, H., & Saroinsong, Y. (2020). Testing the Antioxidant Activity of Sonneratia alba Mangrove Leaf Extract from Tagulandang District, North Sulawesi Using the DPPH Method. Tropical Biopharmaceuticals, 3(1), 79–85. https://doi.org/10.55724/j.biofar.trop.v3i1.260
Ministry of Health of the Republic of Indonesia. (1995). Pharmacopoeia Edition IV. Jakarta: Director General of Drug and Food Control.
Ministry of Health of the Republic of Indonesia. (2000). General Standard Parameters for Medicinal Plant Extracts. Jakarta: Ministry of Health of the Republic of Indonesia.
Dwiningrum, S. J., Hajrah., Rijai, H. R. (2024). Production of Tea Bags Combining Guava Leaves (Psidium Guajava) and Soursop Leaves (Annona Muricata L.) as Antioxidants. Journal Syntax Idea, 6(06).
Hilmarni, H., Rosi, D. H., & Kusuma, A. E. (2021). Isolation and Testing of the Antibacterial Activity of Torbangun Leaf Essential Oil (Plectranthus Amboinicus (Lour.) Spreng against Staphylococcus aureus Bacteria. Journal of Higea Pharmacy, 13(2), 65. https://doi.org/10.52689/higea.v13i2.361
Kaban, V. E., Yusmarlisa, S. (2018). Testing the Activity of Bangun-Bangun Leaves (Plectranthus Amboinicus) Using Ultraviolet-Visible Spectrophotometry. Jurnal Farmasimed (JFM) 1(1).
Leo, R., & Daulay, A. S. (2022). Determination of Vitamin C Content in Vitamin-Fortified Beverages Stored at Various Times Using UV Spectrophotometry. Journal of Health and Medical Science, 1(2), 105–115. https://pusdikra-publishing.com/index.php/jkes/home
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. sci. technol 26, 211-219.
Mu'nisa. (2023). Antioxidants in Plants and Their Role in Degenerative Diseases. Brilian Internasional Surabaya, 91–106. website: www.brilianinternasional.com
Mutmainna Tamrin, Achmad Kadri Ansyori, & Hayatus Sa'adah. (2024). Testing the Antioxidant Activity of Ethanol Extracts from Nyirih Fruit Seeds (Xylocarpus granatum) Using the DPPH Method by UV-Vis Spectrophotometry. Indonesian Journal of Pharmaceutical Research, 6(2), 233–248. https://doi.org/10.33759/jrki.v6i2.527
Nisa, S., Maryono, M., & Dini, I. (2024). Antioxidant Activity of Ethanol and Water Extracts of Breadfruit Leaves (Artocarpus altilis (Park) Using the DPPH (2,2-Diphenyl-1-Picrylhydrazine) Method. Chemica: Journal of Chemistry and Chemistry Education, 25(1), 1. https://doi.org/10.35580/chemica.v25i1.60712
Nurkhasanah, M. A., Si, A., Mochammad, S., Bachri, S., Si, M., Si, D. S., & Yuliani, M. P. (2023). Antioxidants and Oxidative Stress.
Pane, Y. S. (2023). The Potential of Bangun-Bangun Leaf Extract (Coleus amboinicus) as a Herbal Analgesic in Relieving Inflammation. Medan: USU Press.
Silitonga, M. (2021). Various Studies on the Efficacy of Bangun-Bangun Leaves (Plectranthus amboinicus L. Spreng). VII National Webinar on Biology and Its Learning, 301–312.
Suhanda, H. (2022). Troubleshooting in UV-VIS Spectrophotometer Analysis. Indonesian Bright House Association.
Susiloningrum, D., & Mugita Sari, D. E. (2021). Testing the Antioxidant Activity and Determining the Total Flavonoid Content of Temu Mangga Extract (Curcuma Mangga Valeton & Zijp) with Variations in Solvent Concentration. Cendekia Journal of Pharmacy, 5(2), 117–127. https://doi.org/10.31596/cjp.v5i2.148
Trimidianti, W., Faisal, M., & Sastyarina, Y. (2022). Antioxidant Activity Test of Kersen Leaf Decoction (Muntingia calabura L.) using the DPPH (1,1-diphenyl-2-picrylhydrazyl) Method. Proceeding of Mulawarman Pharmaceuticals Conferences.
